Sponsored content brought to you by
Digital PCR (dPCR) earned its reputation by solving a real problem: it let researchers count nucleic acid molecules directly, without leaning on a standard curve. But the method works by assuming something it cannot verify—that each of a limiting number of droplets holds no more than one target molecule. At low concentrations, that assumption mostly holds. Push the input higher, or add a few more targets to the same reaction, and multiple target molecules start sharing individual partitions. At that point, the instrument is no longer counting and is instead estimating using Poisson statistics.
The gap between what dPCR promises and what it can reliably deliver as target input…
Click here to view original post
Click Here to Publish/Feature Your Company or Product News with Biotech Networks
